Role of glypican-3 and heppar-1 in categorization of hepatic space occupying lesions in imaging guided fine needle aspiration cytology

Abstract

Background: Hepatocellular carcinoma (HCC) constitutes of 80% of all primary liver malignancy. Second most common site of metastases is liver. The current study is aimed to diagnose and categorize different primary and metastatic lesions of liver through expression of HepPar-1 and Glypican-3 in FNAC and cell block preparations of materials obtained by ultrasound guided technique.
Materials and Methods : In this prospective study 50 patients of Nil Ratan Sircar Medical College, Kolkata, are included to see the expressions of immunomarkers in neoplastic hepatic lesions.
Results: We got mostly (58%) middle-aged male patients. Eighty percent of patients presented with weight loss. Radiologically, 86% of primary hepatic lesions were solitary. Cirrhotic patients were eight (08). Most of the cases were metastatic carcinomas (72%) and HCC was 22%. Commonest was Grade I. Glypican-3 and HepPar-1 had a sensitivity, specificity and accuracy of 41.6%, 100%, 86.0% and 75%, 97.4%, 92% respectively. The combined sensitivity, specificity and accuracy of both markers were 83.3%, 97.4% and 94.0% respectively.
Conclusion: Combined use of Glypican-3 and HepPar-1 markers are very useful to differentiate between HCC and metastatic hepatic lesions by using ultrasound guided FNAC.