Intestinal carriage and molecular characteristics of carbapenem resistant Escherichia coli and Klebsiella pneumoniae among patients in intensive care units and outpatients in a tertiary care hospital
DOI:
https://doi.org/10.18231/j.pjms.2023.108Keywords:
Carbapenemase, betalactamase NDM1, Gastrointestinal carriage, Outpatients, Intensive Care Units, IndiaAbstract
Introduction: Intestinal carriage of carbapenem-resistant and (CREK) plays an important role in the epidemiology of carbapenemase producers. Less is known about the carriage of CREK among the outpatients when compared to carriage among patients in Intensive Care Units. (ICU) Therefore the present study was performed to detect colonization of CREK among ICU patients and outpatients of our tertiary care hospital.
Materials and Methods: Rectal swabs from ICU patients and stool specimens from outpatients were collected. Identification and antimicrobial susceptibility were performed using Vitek 2 compact system. Screening for CREK was done by two methods and confirmed for carbapenemase production by mCIM. Carbapenemase genes were detected by multiplex polymerase chain reaction.
